Laboratory animal care and development of animal models

• The main activity of animal facility staff is to breed and maintain the laboratory animal colonies in complete compliance with the CPCSEA and in-house animal care principles, rules, regulations and guidelines.
• We have developed various  animal  disease  models  such  as  acute  renal  failure,  chronic  renal  failure,  myocardial infarction,experimental  autoimmune  encephalomyelitis, diabetes,  osteochondral  defect,  breast  cancer, alzheimer’s  disease,  inflammation  induced  anaemia,,  lethal  hemorrhagic  injury,  chronic  wound  excision etc for testing of various biological active molecules.

Stem cell research and therapy

• We have isolated stem cells from various tissue sources viz.fetal kidney, fetal heart and bone marrow. We further tested their therapeutic efficacy in different animal disease models viz. acute renal failure, myocardial infarction, experimental autoimmune encephalomyelitis and global heart failure.
• We are now focusing on the development of novel methods for non viral transfection of cardiac specific transcription factors to induce cardiomyocytes from cardiac fibroblast in ischemic myocardial infraction settings.

miRNA based therapeutics for various diseases

• We are working  to  develop  miRNA  based  therapeutics  for  acute  and  chronic  renal  failure,  and  had intramural  project  on  the  same 
• We evaluated potential of  nanoparticles  based  delivery  of  miRNAs  for  myocardial  repair  and 
• Recently we are interested to unveil the miRNA mediated signalling molecules that regulate the cross talk between autophagy and apoptosis after myocardial infarction

Biomedical Applications of nanotechnology

• WE evaluated potential of bacterial cellulose based bilayered scaffolds in a rat model of osteochondral defects and showed progressive regeneration of cartilage
• We determined anticancer activity of truncated domain of SMAR1 protein (His 5) loaded on Carbon nanospheres (CNS) in mice bearing breast tumours
• We are now studying combination therapy of surface functionalized Lanthanum Strontium Manganese Oxide nanoparticles mediated hyperthermia along with doxorubicin for the treatment of breast cancer

Sayed  N,  Tambe  P,  Kumar  P, Jadhav  S,  Paknikar  KM, Gajbhiye  V.  miRNA  transfection  via poly(amidoamine)-based   delivery   vector   prevents   hypoxia/reperfusion-induced   cardiomyocyte apoptosis. Nanomedicine (Lond). 2020 15 (2):164-181

Ghatpande  NS,  Misar  AV,  Waghole  RJ,Jadhav  SH,  Kulkarni  PP.  Tinospora  cordifolia  protects against  inflammation associated  anemia  by  modulating  inflammatory  cytokines  and  hepcidin

expression in male Wistar rats. Sci Rep. 2019 Jul 29;9(1):10969.

Monsoor  Shaik  M.  Dapkekar  AG, Rajwade  J  M,  Jadhav  S  H,  KowshikM.  (2019)  Antioxidantantibacterial  containing  bilayer  scaffolds  as potential candidates for management of oxidative stress and infections in  wound  healing.  Journal  of  Materials  Science:  Materials  in  Medicine 30:13

Bhagat  PN,  Jadhav  SH,  Chattopadhyay  S, Paknikar   KM.  Carbon nanospheres mediated nuclear delivery of SMAR1 protein (DNA binding domain)  controls  breast  tumor  in  mice  model.  Nanomedicine  (Lond). 2018 Feb;13(4):3532017-0298.

Singh SP, Jadhav SH, Chaturvedi CP, NityanandS. Therapeutic efficacy of multipotent adult progenitor cells versus mesenchymal stem cells in experimental autoimmune   encephalomyelitis.   Regen   Med.   2017 Apr;12(4):377-396. doi: 10.2217/rme-2016-0109

Kumbhar J V, Jadhav S H, Bodas D S, Barhanpurkar-Naik A, Wani M R, Paknikar  K  M,  Rajwade  J  M.  (2017)  In  vitro  and  in  vivo  studies  of  a novel bacterial cellulosebased acellular bilayer nanocomposite scaffold for  the  repair  of  osteochondral  defects.  International  Journal  of Nanomedicine, 12: 6437-6459.5.

Chhabra  H,  Kumbhar  J  V,  Rajwade  J  M,  Jadhav  S,  Paknikar K  M, Jadhav  S,  Bellare  J  (2016)Three-dimensional  scaffold  of  gelatin–poly (methyl  vinyl  ether-alt-maleic  anhydride)  for  regenerative  medicine: Proliferation  and  differentiation  of  mesenchymal  stem  cells

Journal  of Bioactive and Compatible Polymers 31 (3), 273-290

Gupta  AK,  Jadhav  SH,  Tripathy  NK,  NityanandS.  Fetal  Kidney  Cells Can  Ameliorate  Ischemic  Acute  Renal  Failure  in  Rats  through  Their Anti-Inflammatory,  Anti-Apoptotic  and  Anti-Oxidative  Effects.  PLoS One. 2015 Jun 18;10(6):e0131057.

Gupta  AK,  Jadhav  SH,  Tripathy  NK,  Nityanand  S.  Fetal  kidney  stem cells ameliorate cisplatin induced acute renal failure and promote renal angiogenesis.  World J Stem Cells. 2015 May 26;7(4):776-8810.

Garikipati VN, Jadhav S, Pal L, Prakash P, Dikshit M and Nityanand S. Mesenchymal  stem  cells  from  fetal  heart  attenuate  myocardial  injury after infarction: an in vivo serial pinhole gated SPECT-CT study in rats. PLoS One. 2014 27; 9(6 :e100982)

Krynetskaia NF, Phadke MS, Jadhav SH, Krynetskiy EY. Chromatin-associated proteins HMGB1/2 and PDIA3 trigger cellular response to chemotherapy-induced DNA damage. Mol Cancer Ther. 2009 Apr; 8(4):864-72.

S Jadhav, N Krynetskaia, E Krynetskiy Cellular response to anticancer drugs in melanoma cells: inhibition of glyceraldehyde 3-phosphate dehydrogenase results in cell cycle arrest and increased chemoresistance to chemotherapeutic agents Cancer Research 2007 67 (9 Supplement), 3238

Jadhav SH, Sarkar SN, Patil RD, Tripathi HC. Effects of Subchronic Exposure via Drinking Water to a Mixture of Eight Water-Contaminating Metals: A Biochemical and Histopathological Study in Male Rats. Arch Environ Contam Toxicol. 2007 Nov; 53(4):667-77.

Jadhav SH, Sarkar SN, Ram GC, Tripathi HC. Immunosuppressive Effect of Subchronic Exposure to a Mixture of Eight Heavy Metals, Found as Groundwater Contaminants in Different Areas of India, , Drinking Water in Male Rats. Arch Environ Contam Toxicol. 2007 53 (3), 450-458

Jadhav SH, Sarkar, S.N., Kataria, M., Tripathi, H.C. Subchronic exposure to a mixture of groundwater -contaminating metals through drinking water induces oxidative stress in male rats Environmental Toxicology and Pharmacology. Vol. 23, No. 2, pages 205-211 (2007)

Jadhav SH, Sarkar SN, Tripathit HC. Cytogenetic effects of a mixture of selected metals following subchronic exposure through drinking water in male rats. Indian J ExpBiol.2006Dec;44(12):997-1005.

Jadhav SH, Sarkar SN, Aggarwal M, Tripathi HC. Induction of oxidative stress in erythrocytes of male rats subchronically exposed to a mixture of eight metals found as groundwater contaminants in different parts of India. Arch Environ Contam Toxicol.2007Jan;52(1):145-51.

Naik AK, Tandan SK, Dudhgaonkar SP, Jadhav SH, Kataria M, Prakash VR, Kumar D. Role of oxidative stress in pathophysiology of peripheral neuropathy and modulation by N-acetyl cysteine in rats. Eur J Pain. 2006 Oct;10(7):573-9.

Dudhgaonkar SP, Tandan SK, Bhat AS, Jadhav SH, Kumar D. Synergistic anti- inflammatory interaction between meloxicam and aminoguanidine hydrochloride in carrageenan-induced acute inflammation in rats. Life Sci. 2006 Feb 2;78(10):1044-8